Extractable Bacterial Surface Proteins in Probiotic–Host Interaction

Extractable Bacterial Surface Proteins in Probiotic–Host Interaction

Some Gram-positive bacteria, including probiotic ones, are covered with an external proteinaceous layer called a surface-layer. Described as a paracrystalline layer and formed by the self-assembly of a surface-layer-protein (Slp), this optional structure is peculiar. The surface layer per se is conserved and encountered in many prokaryotes. However, the sequence of the corresponding Slp protein...

Bacterial surface proteins and vaccines

Surface-associated proteins play a key role in bacterial physiology and pathogenesis and are the major targets for vaccine development. Recent advances in defining the proteins associated with, and protruding out of, bacterial cells to a high level of accuracy are substantially contributing to accelerating the process of vaccine target identification and development.

Purification Systems Based on Bacterial Surface Proteins

Affinity purification is based on the selective and reversible interaction between two binding partners, of which one is bound to a chromatography matrix and the other may be either a native target protein or a recombinant protein fused with an affinity tag (Cuatrecasas et al. 1968). Recombinant DNA-technology allows straightforward construction of gene fusions to provide fusion proteins with t...

proteomics study of polyethylenimine interaction with cell surface proteins in human colon adenocarcinoma cell line

INTERACTION OF PROTEINS WITH SMALL MOLECULES